GLYCINE; RB X-100UL

Application

Research CategoryNeuroscience

This Anti-Glycine Antibody is validated for use in IH for the detection of Glycine.

Research Sub CategoryNeurotransmitters & Receptors

Immunohistochemistry: 1:500-1:2,500 by PAP (see suggested protocol).

Optimal working dilutions must be determined by the end user.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Glycine-gluteraldehyde-BSA

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit antiserum. Liquid with 0.05% sodium azide.

Specificity

Glycine

The cross-reactivities were determined using either ELISA or RIA techniques, at concentration/unconjugated or conjugated amino acid concentration at half displacement.



Compound Cross-reactivity

Glycine-G-BSA 1

Beta Alanine-G-BSA 1/100

GABA-G-BSA 1/500

Taurine-G-BSA 1/1000

Aspartate-G-BSA 1/20000

Glutamate-G-BSA ﹤1/20000

Glycine ﹤1/50000



The antisera was also tested for specificity using the free-floating PAP technique on rat spinal cord.



Abbreviations:

(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.